Відео

what is the difference between relative protein quantification and absolute protein quantification?

Thanks for sharing it.

Having trouble finding the follow up videos for this series, do they in fact exist?

Why only 240p? Unfortunately looks like pixel salad

🙏🏼🙏🏼

thx man

lol! Great ad

Nikolai, is this a better approach in relation to Nautilus Biotechnology platform? How do you expect the throughput and quality of protein identification?

based, excelent video.

The first article about patch-clamp integration with MS / proteomics: O. Gradov and M. Gradova, "MS-patch-clamp or the possibility of mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics", Advances in Biochemistry 3 (2015), 66-71. This paper was cited in: Zhu, Hongying, Qianqian Li, Tiepeng Liao, Xiang Yin, Qi Chen, Ziyi Wang, Meifang Dai et al. "Metabolomic profiling of single enlarged lysosomes." Nature Methods 18, no. 7 (2021): 788-798 (experimental paper, realized so beautiful ideas) and Zhang, Linwen, and Akos Vertes. "Single‐cell mass spectrometry approaches to explore cellular heterogeneity." Angewandte Chemie International Edition 57, no. 17 (2018): 4466-4477. {Zhang, Linwen, and Akos Vertes. "Einzelzell‐Massenspektrometrie zur Untersuchung zellulärer Heterogenität." Angewandte Chemie 130, no. 17 (2018): 4554-4566}. The first proposal of this approach (unrealized in destroyed russian infrastructure under the pressing of totalitarian science organization in Russia): Gradov O.V., Gradova M.A. (2014). On the possibility of "MS-patch-clamp" or mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics. In: Proc. Int. Workshop “Structure and Functions of Biomembranes” (29 Sept. - 3 Oct. 2014; Moscow Institute of Physics and Technology), 105-106. I am very glad and happy to see so beautiful works in the Prof. Meni Wanunu presentation. I think, that it is very perspective research branch. Best regards, O.V. Gradov, senior reseracher.

Fantastic presentation & transformative technology )

What biology are we learning? The section starts at 16:27

Fantastic presentation!

A primer and current trends in single-molecule proteomics: 22:30

Current trends in single-cell mass spec proteomics: 8:54

Introduction to SCP2023 and logistics: 0:33

The first article about patch-clamp integration with MS / proteomics: O. Gradov and M. Gradova, "MS-patch-clamp or the possibility of mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics", Advances in Biochemistry 3 (2015), 66-71. This paper was cited in: Zhu, Hongying, Qianqian Li, Tiepeng Liao, Xiang Yin, Qi Chen, Ziyi Wang, Meifang Dai et al. "Metabolomic profiling of single enlarged lysosomes." Nature Methods 18, no. 7 (2021): 788-798 (very beautiful experimental paper, realized so original ideas) and Zhang, Linwen, and Akos Vertes. "Single‐cell mass spectrometry approaches to explore cellular heterogeneity." Angewandte Chemie International Edition 57, no. 17 (2018): 4466-4477. {Zhang, Linwen, and Akos Vertes. "Einzelzell‐Massenspektrometrie zur Untersuchung zellulärer Heterogenität." Angewandte Chemie 130, no. 17 (2018): 4554-4566}. The first proposal of this approach (unrealized in destroyed russian infrastructure under the pressing of totalitarian science organization in Russia): Gradov O.V., Gradova M.A. (2014). On the possibility of "MS-patch-clamp" or mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics. In: Proc. Int. Workshop “Structure and Functions of Biomembranes” (29 Sept. - 3 Oct. 2014; Moscow Institute of Physics and Technology), 105-106. I am very glad and happy to see so beautiful works in the Slavov Lab, which can be intepreted as one of the most respectable laboratory in this branch of science (or R&D). Best regards, O.V. Gradov.

The first article about patch-clamp integration with MS / proteomics: O. Gradov and M. Gradova, "MS-patch-clamp or the possibility of mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics", Advances in Biochemistry 3 (2015), 66-71. This paper was cited in: Zhu, Hongying, Qianqian Li, Tiepeng Liao, Xiang Yin, Qi Chen, Ziyi Wang, Meifang Dai et al. "Metabolomic profiling of single enlarged lysosomes." Nature Methods 18, no. 7 (2021): 788-798 (experimental paper, realized so beautiful ideas) and Zhang, Linwen, and Akos Vertes. "Single‐cell mass spectrometry approaches to explore cellular heterogeneity." Angewandte Chemie International Edition 57, no. 17 (2018): 4466-4477. {Zhang, Linwen, and Akos Vertes. "Einzelzell‐Massenspektrometrie zur Untersuchung zellulärer Heterogenität." Angewandte Chemie 130, no. 17 (2018): 4554-4566}. The first proposal of this approach (unrealized in destroyed russian infrastructure under the pressing of totalitarian science organization in Russia): Gradov O.V., Gradova M.A. (2014). On the possibility of "MS-patch-clamp" or mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics. In: Proc. Int. Workshop “Structure and Functions of Biomembranes” (29 Sept. - 3 Oct. 2014; Moscow Institute of Physics and Technology), 105-106. I am very glad and happy to see so beautiful works in the Slavov Lab, which can be intepreted as one of the most respectable laboratory in this branch of science (or R&D). Best regards, O.V. Gradov.

The first article about patch-clamp integration with MS / proteomics: O. Gradov and M. Gradova, "MS-patch-clamp or the possibility of mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics", Advances in Biochemistry 3 (2015), 66-71. This paper was cited in: Zhu, Hongying, Qianqian Li, Tiepeng Liao, Xiang Yin, Qi Chen, Ziyi Wang, Meifang Dai et al. "Metabolomic profiling of single enlarged lysosomes." Nature Methods 18, no. 7 (2021): 788-798 (experimental paper, realized so beautiful ideas) and Zhang, Linwen, and Akos Vertes. "Single‐cell mass spectrometry approaches to explore cellular heterogeneity." Angewandte Chemie International Edition 57, no. 17 (2018): 4466-4477. {Zhang, Linwen, and Akos Vertes. "Einzelzell‐Massenspektrometrie zur Untersuchung zellulärer Heterogenität." Angewandte Chemie 130, no. 17 (2018): 4554-4566}. The first proposal of this approach (unrealized in destroyed russian infrastructure under the pressing of totalitarian science organization in Russia): Gradov O.V., Gradova M.A. (2014). On the possibility of "MS-patch-clamp" or mass spectrometry hybridization with patch-clamp setups for single cell metabolomics and channelomics. In: Proc. Int. Workshop “Structure and Functions of Biomembranes” (29 Sept. - 3 Oct. 2014; Moscow Institute of Physics and Technology), 105-106. I am very glad and happy to see so beautiful works in the Slavov Lab, which can be intepreted as one of the most respectable laboratory in this branch of science (or R&D).

It's important to keep in mind that the purpose of explaining something is to help others understand, not to showcase our own understanding. By using simple language and clear explanations, we can make the information more accessible and easier to understand for everyone. It's also important to remember that not everyone has the same level of knowledge or expertise, so we should tailor our explanations to our audience. Effective communication is about helping others learn and grow, not about impressing them with our own intelligence

Interesting perspective coming from an elitist lab

13:09

3:25 theories 9:45 addiction 13:00 patch punch

2:55 8:20

Ok this is def one of the more INTERPRET-ABLE vids by you

What you offer is really great. Maybe I cannot fully understand everything because it is not my specialty, but I am really interested in mass spectrometry and what you offer. Thank you You are the best

For more information on this method see: scp.slavovlab.net/nPOP and genomebiology.biomedcentral.com/articles/10.1186/s13059-022-02817-5

this is a wonder presentation.

2:30 is hilarious

Thanks ever so much for uploading this. Very interesting talk and what an amazing technique.

Mode details can be found in Derks, J. et al. Increasing the throughput of sensitive proteomics by plexDIA. Nat Biotechnol (2022). doi.org/10.1038/s41587-022-01389-w. The search is described in the methods section www.nature.com/articles/s41587-022-01389-w#Sec12

where are the .pipeline files that's mentioned in the tutorial?

Thank you, Sir. It is much needed. Can you make lecture on plant metabolite data analysis? Please do the needful. Thank you

Slides are available at: plexdia.slavovlab.net/mass-spec/Searching-plexDIA-data-with-DIA-NN.pdf

Such a low sample size, did you all experimentally validate results? You can shapeshift scRNAseq data into a binary dataframe and compare with bulk for ratios of variance according to disease, prognosis, or treatment

Andrew Leduc described the droplet sample preparation in more details at the workshop of the 4th single-cell proteomics conference: ua-cam.com/video/DJ1U_KpMNcY/v-deo.html

Detecting and quantifying _post-translational modifications_ (PTMs) in single cells: 27:30

Tangible prospects for single-cell proteomics within sight: 4:32. See also pubs.acs.org/doi/10.1021/acs.jproteome.1c00639?ref=pdf

Was APP and tau differentially changed in the PC-MS data?

Hi Nikolai, is the full video available? I think this one cuts out in the middle.

Dear Prof. Slavov, was wondering if you could upload more talks from the conference? Thanks :)

following up on the question around 39:00-40:00 mins, does that mean that the mass of TMTs are much larger than the mass of peptides they bind to?

really interesting the simulated problem, thanks for the video!

40:04 showing from 221 to 1056 protein groups

Excellent presentation Dr., my question is the following, how do you cure your raw data of a biological replica with the other 2? To suggest an increase or decrease in the abundance of some protein compared to the other phenotype (control).

What is the difference between SCoPE-MS and SCoPE 2? saw that you were the main author for the paper just recently published too

Is the video on Imputation coming out soon? Or could you suggest some good resources? Imputation is a topic of debate in our lab.

Wow! This is super interesting. I carried out PatchSeq recently and would be great to carry our single-cell proteomics

Thanks twitter.com/slavov_n?s=20 for the Beautiful presentation of the fact that proteomic space is not only more relevant in terms of interesting #Biology, it is also more accurate ua-cam.com/video/5nV9zbF9DT0/v-deo.html right down to the level of SINGLE Cell!! Even the Spearman<Pearson ;) WTH #SingleCell

Such a clear and well explained presentation ! So happy I could attend to SCP 2020 this summer!